ScienCell's GeneQuery™ Human Smooth Muscle Cell Biology qPCR Array Kit (GQH-SMC) is designed to facilitate gene expression profiling of 88 key genes involved in human smooth muscle cell (SMC) biology, such as vascular SMC contraction, SMC phenotypic switching, and the extracellular matrix (ECM) synthesis. Genes implicated in selected SMC related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

• SMC markers: ACTA2, CDH5, CALD1, HEXIM1, HRH2, MLNR, TAGLN, DES, CSPG4, SMTN, MKL1/2

• Vascular SMC contraction: ANO1, ADRA1s, NPR1/2, GNAs, MYLKs, ADORA2s, EDNRA, MYH11, MYLs

• SMC phenotypic switching: MYH11, MEF2B, SRF, ELK1, MYOCD, GATA6, MKL1/2, RHOA, TGFB1, NOTCH1/3, HEY2
• SMC heterogeneity: ACTA2, TAGLN, CNN2, MYH11, OGN, AQP1, PLN, MGP, SPP1, KDR, SMTN, VCL, DES
• ECM synthesis: LAMA5, collagens, ELN, OGN, MGP, SPP1, FGA/B/G
• Genes implicated in
• systemic hypertension: PTGIS, AGT, ADD1, AGTR1, GNB3, NOS3
• achalasia: NOS1, AAAS, GMPPA, GUCY1A3, HLA-DQA1/B1
• bronchospasm/asthma: HNMT, IL13, CCL11, ADRB2, ALOX5, SCGB3A2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Smooth Muscle Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development