The human kidney is frequently exposed to drugs and toxic compounds, which can result in nephrotoxicity. A drug’s uptake and clearance from the kidney largely relies on a subset of membrane transporters such as organic anion transporters (OAT) 1/3, organic cation transporter (OCT) 2, and multidrug and toxin extrusion proteins (MATE) 1/2. In 2D monolayer culture, primary renal epithelial cells rapidly lose the expression of OAT1/3, OCT2, and MATE1/2, limiting the accurate redictability of a drug’s effect [1]. ScienCell has developed a 3D renal tubule formation kit (3D-RTF) using human primary renal cortical epithelial cells isolated from normal adult kidney tissue, which are then embedded in Collagen I extracellular matrix. We demonstrated that renal tubule structures are formed in a robust and reproducible manner in a 3D collagenembedded model using the 3D-RTF. 3D cultured renal epithelial cells had elevated expression levels of xenobiotic transporters such as OAT1/3, and MATE1/2 (see Figure 1 and 2). The 3DRTF provides a useful in vitro tool for accurately predicting a drug’s effect on kidney function.

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