Lymphatic Mononuclear Cells (LMC) are located in the lymph nodes and perform vital functions for the innate and adaptive immune system [1]. Primary LMC are a mixed population of single nucleus cells which include T-cells (helper T-cells, cytotoxic T-cells, gamma delta T-cells), Bcells, and natural killer (NK) cells. Primary rat LMC (MLMC) can be used to study the innate and adaptive immune system. RLMC from ScienCell Research Laboratories are isolated from healthy adult rat lymph nodes. RLMC are depleted of erythrocytes, cryopreserved immediately after isolation, and delivered frozen. RLMC are a mixed population of cells that include T-cells, B-cells, and NK cells. Each vial contains at least 10 million cells in 1 ml volume. RLMC are quality control tested for viability. RLMC are negative for mycoplasma, bacteria, yeast, and fungi. RLMC can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture. Recommended Medium It is recommended to use HematoGro Medium (HemGM, Cat. #5501) for short-term maintenance of RLMC in vitro.