Our adipogenesis differentiation medium has been specifically developed and optimized for in vitro mesenchymal stem cell adipogenesis study. Generally, with this medium, we obtain >35% mature adipocytes from human bone marrow, >50% from rat bone marrow, and >60% from human mesenchymal stem cell isolated from adipose tissue. Efficiency of adipogenic differentiation depends on the quanlity of the mesenchymal stem cells. Mesenchymal stem cell Adipogenic Differentiation Medium (MADM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. Components MADM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of mesenchymal stem cell adipogenic differentiation supplement (MADS, Cat. No. 7542-5), 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503). Note: MADS may be slightly hazy; Haziness does not affect cell culture and differentiation efficiency.
1.) Moniri MR, Sun XY, Rayat J, Dai D, Ao Z, He Z, Verchere CB, Dai LJ, Warnock GL. (2012) "TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells." Cancer Gene Ther. 19: 652-8.
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