ScienCell's GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit (GQH-CCN) is designed to facilitate gene expression profiling of 40 selected cyclins and cyclin-dependent kinases (CDKs). CDKs are a family of small enzymes required for cell cycle progression and cell division, by means of phosphorylation of their substrates. CDKs must bind to cyclins to become fully activated. Cyclin/CDK complexes regulate cell cycle checkpoints and are the driving force of cell cycle progression. Brief examples of how included genes may be grouped are shown below:
- Cyclins: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY
- CDKs: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
CCNA1 |
CCND3 |
CCNI2 |
CDK2 |
CDK11A |
β-actin |
CCNA1 |
CCND3 |
CCNI2 |
CDK2 |
CDK11A |
β-actin |
|
B |
CCNA2 |
CCNE1 |
CCNK |
CDK3 |
CDK12 |
GAPDH |
CCNA2 |
CCNE1 |
CCNK |
CDK3 |
CDK12 |
GAPDH |
|
C |
CCNB1 |
CCNE2 |
CCNL1 |
CDK4 |
CDK13 |
LDHA |
CCNB1 |
CCNE2 |
CCNL1 |
CDK4 |
CDK13 |
LDHA |
|
D |
CCNB2 |
CCNF |
CCNL2 |
CDK5 |
CDK15 |
NONO |
CCNB2 |
CCNF |
CCNL2 |
CDK5 |
CDK15 |
NONO |
|
E |
CCNB3 |
CCNG1 |
CCNO |
CDK6 |
CDK16 |
PPIH |
CCNB3 |
CCNG1 |
CCNO |
CDK6 |
CDK16 |
PPIH |
|
F |
CCNC |
CCNG2 |
CCNT1 |
CDK7 |
CDK17 |
GDC |
CCNC |
CCNG2 |
CCNT1 |
CDK7 |
CDK17 |
GDC |
|
G |
CCND1 |
CCNH |
CCNY |
CDK8 |
CDK19 |
PPC |
CCND1 |
CCNH |
CCNY |
CDK8 |
CDK19 |
PPC |
|
H |
CCND2 |
CCNI |
CDK1 |
CDK10 |
CDK20 |
NTC |
CCND2 |
CCNI |
CDK1 |
CDK10 |
CDK20 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK063 |
| 产地 | 美国 |
| 缩写 | GQH-CCN |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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