ScienCell's GeneQuery™ Human Neuronal Cell Biology qPCR Array (GQH-NEU) profiles 88 key genes involved in normal neuron function. Neurons are responsible for processing and transmitting information throughout the body via electric potentials. They are a core component of the central nervous system and specialized neurons are key elements of the sensory and motor units. Below are brief examples of how included genes may be grouped according to their function in neuronal cell biology:
- Neurotransmission: ABAT, GABRB1, GRM3, SLC32A1, VAMP2
- Axonal guidance: EFNA2, ROBO1, NTN1, NRP1, SLIT1
- Synapse formation and maintenance: DLG2, NLGN3, SYN1, SYP, DLG4
- Neurite outgrowth: GLDN, GPRIN1, NRCAM, RTN4, SEMA3G
- Differentiation: BMP2, EVX1, GNL3, SMARCA4, PROM1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Neuronal Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABAT |
EFNA2 |
EPHA7 |
GABRB1 |
GRM7 |
NFASC |
NRP2 |
PLCG1 |
ROBO2 |
SEMA3F |
SYP |
β-actin |
|
B |
ABCG2 |
EFNA5 |
EPHB1 |
GAD1 |
HRAS |
NGF |
NTF3 |
PLCG2 |
RTN4 |
SEMA3G |
TUBB3 |
GAPDH |
|
C |
BDNF |
EFNB1 |
EPHB2 |
GAD2 |
ITGB1 |
NGRN |
NTF4 |
PROM1 |
RTN4R |
SLC32A1 |
UNC5A |
LDHA |
|
D |
BMP2 |
EFNB2 |
EVX1 |
GFAP |
KRAS |
NLGN3 |
NTN1 |
RAB11A |
S100B |
SLC6A1 |
UNC5B |
NONO |
|
E |
CLTA |
ENO2 |
FZD1 |
GLDN |
MAP2 |
NOTCH1 |
NTRK1 |
RAB5A |
SEMA3A |
SLIT1 |
UNC5C |
PPIH |
|
F |
DCC |
EPHA1 |
GABBR1 |
GNL3 |
NEDD4L |
NOTCH2 |
NTRK2 |
RAB7A |
SEMA3C |
SLIT2 |
VAMP2 |
GDC |
|
G |
DLG2 |
EPHA2 |
GABBR2 |
GPRIN1 |
NES |
NRCAM |
NTRK3 |
RBFOX3 |
SEMA3D |
SMARCA4 |
VAV2 |
PPC |
|
H |
DYNC1H1 |
EPHA5 |
GABRA2 |
GRM3 |
NEUROD1 |
NRP1 |
PDGFA |
ROBO1 |
SEMA3E |
SYN1 |
VAV3 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK078 |
| 产地 | 美国 |
| 缩写 | GQH-NEU |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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