Pericyte Medium-mouse (PM-m), when used with Pericyte Growth Supplement (PGS-m, Cat #1282) and 10mL FBS is a complete medium designed for optimal growth of normal mouse pericytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mouse pericytes in vitro.

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Question:

Dear Sir, Thanks for the information about mouse pericytes medium. Since I am new to culturing pericytes, I would like to know what kind of coating agents ( like Collagen type I/ IV / gelating/ Poly lysine ?) are used in the plates for culturing them. Kindly let me know. Thanks Malini

Answers:

we recommend Poly lysine