| 货号 | 1252 |
| 产地 | 美国 |
| 缩写 | PGS |
| 规格 | 5ml |
| 用途 | 科研 |
| 储存 | -20度 |
| 运输 | 胶冰 |
1. Question:
Recently I was attempting to isolate pericytes from mouse brain tissue, following established protocols I finally attempted to passage cells in new collagen coated wells and a 10cm dish in PGM with PGM supplement for mouse. My concern was the protocol didn't call for additional serum in the PGM --- does the supplement provide for this or is it an omission in the protocol I used? Reason being I see very few cells growing after incubation. I'm just troubleshooting before I use transgenic mice vital to the research.
Answers:
Our recommended medium is #1231 PM-m (Pericyte Medium-mouse), which does come with 10mL FBS in a separate tube. In addition, we recommend using poly-L-lysine to coat culture flasks.
2. Question:
As the product description says: this product is designed for the optimal growth of normal human pericytes in vitro. I want to know if this product can be used for the culture of rat or mouse pericytes in vitro? Thanks.
Answers:
Yes
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