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周细胞生长添加物 PGS
Pericyte Growth Supplement
品牌:
ScienCell
货号:
1252
价格:
$71
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Pericyte Growth Supplement (PGS) is a medium supplement designed for the optimal growth of normal human pericytes in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human pericytes. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of normal human pericytes in vitro. The supplement is designed as an additive for pericyte medium (PM, Cat. No. 1201) and should be used in conjunction with that medium.
货号1252
产地美国
缩写PGS
规格5ml
用途科研
储存-20度
运输胶冰
No Publication available at this time

1. Question:

Recently I was attempting to isolate pericytes from mouse brain tissue, following established protocols I finally attempted to passage cells in new collagen coated wells and a 10cm dish in PGM with PGM supplement for mouse. My concern was the protocol didn't call for additional serum in the PGM --- does the supplement provide for this or is it an omission in the protocol I used? Reason being I see very few cells growing after incubation. I'm just troubleshooting before I use transgenic mice vital to the research.


   Answers:

Our recommended medium is #1231 PM-m (Pericyte Medium-mouse), which does come with 10mL FBS in a separate tube. In addition, we recommend using poly-L-lysine to coat culture flasks. 

2. Question:

As the product description says: this product is designed for the optimal growth of normal human pericytes in vitro. I want to know if this product can be used for the culture of rat or mouse pericytes in vitro? Thanks.

Answers:

Yes

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细胞生长添加物
细胞生长添加物
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