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Human CD105 ELISA Kit
Human CD105 ELISA Kit
品牌:
ScienCell
货号:
EK0644
价格:
$485
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CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-β (TGF-β) receptor complex and binds TGF-β1 with high affinity. CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons. The protein consists of a homodimer of 180 kDa with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation. Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggests a persisting post-mitotic activation in advanced stage of neovascular tissue. The standard product used in this kit is the extracellular region of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85 kDa.

ScienCell's human CD105 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CD105-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human CD105 in the sample that is captured on the strips.
货号EK0644
产地美国
缩写hCD105-ELISA
规格96 tests
用途科研
储存4度
运输胶冰
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