ScienCell's GeneQuery™ human neural transmission and membrane trafficking qPCR array kit (GQH-NTM) is designed to facilitate gene expression profiling of key genes involved in (i) human neurotransmission; (ii) membrance trafficking; and (iii) related diseases such as generalized anxiety disorder, DDC deficiency, TH deficiency, and SPR deficiency. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Neural Transmission and Membrane Trafficking qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ADRA1A |
BDNF |
CHRNA5 |
DRD2 |
GABRA2 |
GABRG3 |
GRIK1 |
GRM3 |
HTR2A |
OPTN |
SNAP25 |
β-actin |
|
B |
ADRA1D |
BTD |
CHRNA6 |
DRD4 |
GABRA4 |
GABRQ |
GRIK2 |
GRM4 |
HTR2C |
OTC |
SOD1 |
GAPDH |
|
C |
ADRA2A |
CHMP2B |
CHRNA7 |
DRD5 |
GABRA5 |
GABRR2 |
GRIK4 |
GRM6 |
HTR3A |
PTS |
SPR |
LDHA |
|
D |
ADRB2 |
CHRM1 |
CHRNE |
FIG4 |
GABRB1 |
GCH1 |
GRIK5 |
GRM7 |
HTR4 |
QDPR |
TARDBP |
NONO |
|
E |
ADRB3 |
CHRM4 |
COMT |
FUS |
GABRB3 |
GH1 |
GRIN2A |
HTR1A |
HTR7 |
RAB10 |
TH |
PPIH |
|
F |
AKR1B1 |
CHRM5 |
DBH |
G6PD |
GABRE |
GRIA1 |
GRIN2B |
HTR1B |
LLGL1 |
SLC6A2 |
TPH1 |
GDC |
|
G |
ALDH5A1 |
CHRNA3 |
DHFR |
GABBR1 |
GABRG1 |
GRIA2 |
GRIN2C |
HTR1D |
LPL |
SLC6A3 |
VAPB |
PPC |
|
H |
ALS2 |
CHRNA4 |
DRD1 |
GABBR2 |
GABRG2 |
GRIA3 |
GRM1 |
HTR1F |
MAOA |
SLC6A4 |
VCP |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK008 |
| 产地 | 美国 |
| 缩写 | GQH-NTM |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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