ScienCell's GeneQuery™ Human Pericyte Biology qPCR Array Kit (GQH-PER) profiles 88 key genes involved in pericyte function during angiogenesis and in immunity. In vasculature, pericytes function as mural cells on small blood vessels and they help maintain the blood-brain barrier (BBB), an integral homeostatic brain function. Pericytes also possess macrophage-like properties when functioning in the immune response. Defects in pericyte function can result in BBB breakdown and aberrant vasculature formation. Below are brief examples of how included genes may be grouped according to their function in pericyte biology:
- Angiogenesis: VEGFA, HIF1A, JAG1, CTGF, ANGPT2
- BBB maintenance: ABCC9, VCAM1, OCLN, ICAM1, DLK1
- Inflammation: RIPK2, TLR3, CD74, CXCL9, APOL3
- Scavenging: FCGR2A, HLA-E, ITGAM, C3, CD36
- Markers: CSPG4 (NG2), PDGFRB, MCAM, RGS5, CD248
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Pericyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABCC9 |
APOL3 |
CD4 |
CSPG4 |
CXCL9 |
EGFR |
HIF1A |
ITGAM |
NOTCH1 |
PTGS2 |
TLR3 |
β-actin |
|
B |
ACTA2 |
ARNT2 |
CD40 |
CTGF |
CXCR3 |
EPHA2 |
HLA-DMA |
JAG1 |
NOTCH3 |
RET |
TNC |
GAPDH |
|
C |
ADM |
C1R |
CD47 |
CXCL1 |
DES |
ERBB2 |
HLA-E |
KCNJ8 |
NOX4 |
RGS5 |
TNFRSF10D |
LDHA |
|
D |
AGTR1 |
C3 |
CD68 |
CXCL10 |
DLK1 |
FCAR |
ICAM1 |
MCAM |
OCLN |
RIPK2 |
TNFRSF14 |
NONO |
|
E |
ANGPT1 |
CCL2 |
CD74 |
CXCL11 |
EDN1 |
FCGR1A |
IL32 |
MMP2 |
PDGFRB |
SOD2 |
TPM1 |
PPIH |
|
F |
ANGPT2 |
CCL8 |
CDH2 |
CXCL12 |
EDNRA |
FCGR2A |
IL6 |
MYLK |
PECAM1 |
TGFB1 |
VCAM1 |
GDC |
|
G |
ANPEP |
CD248 |
CREB5 |
CXCL2 |
EDNRB |
GJB6 |
IRF1 |
NFKB1 |
PRKAA1 |
TGFB3 |
VEGFA |
PPC |
|
H |
APOL2 |
CD36 |
CREBBP |
CXCL8 |
EFNB2 |
HBEGF |
ITGA5 |
NOS2 |
PSMB8 |
TGFBR1 |
VEGFC |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK081 |
| 产地 | 美国 |
| 缩写 | GQH-PER |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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