星形胶质细胞培养基 AM
Astrocyte Medium
品牌：
ScienCell
货号：
1801
价格：
$127
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星形胶质细胞培养基是为正常人类星形胶质细胞体外培养设计的适于其生长的培养基。经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类星形胶质细胞体外培养中的增殖和生长，并为其达到理想营养平衡状态提供数量上和质量上的保证。
| 货号 | 1801 |
| 产地 | 美国 |
| 缩写 | AM |
| 规格 | 500ml |
| 用途 | 科研 |
| 储存 | 4度，-20度 |
| 运输 | 胶冰 |
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2.) Vijayaraghavan S, Karami A, Aeinehband S, Behbahani H, Grandien A, Nilsson B, Ekdahl KN, Lindblom RP, Piehl F, Darreh-Shori T. (2013) "Regulated Extracellular Choline Acetyltransferase Activity - The Plausible Missing Link of the Distant Action of Acetylcholine in the Cholinergic Anti-Inflammatory Pathway." PLoS One. 8: e65936
3.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) 'Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration.'
4.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) "Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration." Transloncol. 6: 638-48.
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6.) Robinson JP, Vanbrocklin MW, Lastwika KJ, McKinney AJ, Brandner S, Holmen SL. (2010) 'Activated MEK cooperates with Ink4a/Arf loss or Akt activation to induce gliomas in vivo.'
7.) Robinson JP, Vanbrocklin MW, Lastwika KJ, McKinney AJ, Brandner S, Holmen SL. (2010) "Activated MEK cooperates with Ink4a/Arf loss or Akt activation to induce gliomas in vivo." Oncogene. 30: 1341-50.
1、Question:
Is it possible to freeze aliquots of complete medium to avoid that growth factors will become unstable at 4°C if the medium stays open longer than 1 month?thanks
Answers:
From our FAQ section: We do not recommend subjecting growth supplements to freeze-thaw, as this can result in reduced activity of components and the potential for components to irreversibly precipitate. Additionally, We do not recommend freezing media, as this can result in irreversible precipitation of media components.
Is it possible to freeze aliquots of complete medium to avoid that growth factors will become unstable at 4°C if the medium stays open longer than 1 month?thanks
Answers:
From our FAQ section: We do not recommend subjecting growth supplements to freeze-thaw, as this can result in reduced activity of components and the potential for components to irreversibly precipitate. Additionally, We do not recommend freezing media, as this can result in irreversible precipitation of media components.
2、Question:
Hi, is it recommended to grow HA in 2% FBS (10ml of FBS provided in the kit)? I am working with Astrocytes for the first time and wanted to make sure that I am doing everything right. So basically to make the complete medium, I add 2% FBS, 1% P/S and 1% growth supplement? Thanks!
Answers:
?Thanks for asking! Your process is correct.
特殊培养基
特殊培养基
