Lymphatic Mononuclear Cells (LMC) are located in the lymph nodes and perform vital functions for the innate and adaptive immune system [1]. Primary LMC are a mixed population of single nucleus cells which include T-cells (helper T-cells, cytotoxic T-cells, gamma delta T-cells), Bcells, and natural killer (NK) cells. Primary mouse LMC (MLMC) can be used to study the innate and adaptive immune system. MLMC from ScienCell Research Laboratories are isolated from healthy adult C57BL/6 mouse lymph nodes. MLMC are depleted of erythrocytes, cryopreserved immediately after isolation, and delivered frozen. MLMC are a mixed population of cells that include T-cells, B-cells, and NK cells. Each vial contains at least 10 million cells in 1 ml volume. MLMC are quality control tested for viability. MLMC are negative for mycoplasma, bacteria, yeast, and fungi. MLMC can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture. Recommended Medium It is recommended to use HematoGro Medium (HeGM, Cat. #5501) for short-term maintenance of MLMC in vitro.