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Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction between acetyl coenzyme A (acetyl CoA) and oxaloacetic acid to form citric acid and CoA with a thiol group (CoA-SH). This colorimetric assay is based on the reaction between 5’, 5’-Dithiobis 2-nitrobenzoic acid (DTNB) and CoA-SH to form TNB, which exhibits an absorbance maximum at 412 nm. The intensity of the absorbance is proportional to the citrate synthase activity. This enzyme is an exclusive marker of the mitochondrial matrix.
货号 | 8318 |
产地 | 美国 |
缩写 | CS |
规格 | 50 tests in cuvette or 250 tests in 96-well plate |
用途 | 科研 |
储存 | -20度 |
运输 | 干冰 |
细胞检测试剂盒
细胞检测试剂盒