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Mycoplasma, the smallest and self-replicating prokaryotes, are common contaminants of primary cells and cell lines. Mycoplasma contamination can affect cell growth, morphology and metabolism. Mycoplasma are able to pass through 0.2 µm filters due to their small size. Furthermore, unlike bacteria and fungi, mycoplasma contamination is resistant to commonly used antibiotics, and can not be identified visually. Due to the difficulties in detecting mycoplasma, cells should be regularly screened to control mycoplasma contamination. Conventional screening methods include microbiological culture, fluorescent DNA staining, and biochemical detection methods, and are typically time consuming and lack sensitivity. As a result, polymerase chain reaction (PCR) based methods have been developed as a quick and convenient detection of low level mycoplasma infection, while providing high sensitivity and specificity.
Alignment studies of mycoplasma 16S rRNA sequences revealed the existence of highly conserved sequences. Primers targeting these sequences allow for the specific amplification of the mycoplasma DNA fragment. ScienCell™ Mycoplasma PCR Detection Kit is a 16S rRNA-based PCR assay consisting of 2×PCR master mix (which includes PCR buffer, DNA polymerase and dNTPs), mycoplasma primer set, and a positive control, which is noninfectious genomic DNA (gDNA) of M. fermentans. The genus-specific primer set recognizes the five typical contaminating mycoplasma species (i.e., M. hyorhinis, M. arginini, M. orale, M. fermentans, and A. laidlawi), which account for 98% of contamination, as well as members of the genera Ureaplasma, Spiroplasma, and Acholeplasma, which account for the remaining 2% of contamination. Eukaryotic and bacterial DNA is not amplified by this kit. The size of the expected band is 280 bp.
货号 | 8208 |
产地 | 美国 |
缩写 | MYCO |
规格 | 100 Tests |
用途 | 科研 |
储存 | -20°C |
运输 | 干冰 |
细胞检测试剂盒
细胞检测试剂盒
货号 | 产品名称 | 规格 | 价格 | 指令 | |
8258 | 碱性磷酸酶活力检测试剂盒 ALP | 500 tests | $412.00 | + | |
8268 | 线粒体分离试剂盒 MITOISO | 100 tests | $348.00 | + | |
8278 | 细胞色素C氧化酶检测试剂盒 COX | 100 tests | $376.00 | + | |
8278 | 细胞色素C氧化酶检测试剂盒 COX | 100 tests | $376.00 | + | |
8288 | 碱性磷酸酶染色试剂盒(红) ALPr | 500 tests | $468.00 | + | |
8298 | 人间充质干细胞脂肪形成检测试剂盒 HMSC-A PCR | 50 reactions | $337.00 | + | |
8308 | L-乳酸盐检测试剂盒 LAC | 100 tests | $141 | + | |
8318 | 柠檬酸合成酶检测试剂盒 CS | 50 tests in cuvette or 250 tests in 96-well plate | $497.00 | + | |
8318 | 柠檬酸合成酶检测试剂盒 CS | 50 tests in cuvette or 250 tests in 96-well plate | $497.00 | + | |
8328 | 人间充质干细胞软骨形成检测试剂盒 HMSC-C PCR | 50 reactions | $337.00 | + | |
8338 | 谷氨酸检测试剂盒 GLU | 100 tests | $432.00 | + | |
8358 | 谷氨酸脱氢酶检测试剂盒 GDH | 100 tests | $432.00 | + | |
8368 | NAD/NADH检测试剂盒 NAD | 100 tests | $435.00 | + | |
8368 | NAD/NADH检测试剂盒 NAD | 100 tests | $435.00 | + | |
8378 | 阿尔新蓝染色试剂盒 ABlue | 100 tests | $156.00 | + | |
8388 | 储液瓶 传统风格 125ml 45mm颈尺寸 带盖 灭菌 | 100 tests | $384.00 | + | |
8398 | 葡萄糖6磷酸酶检测试剂盒 G6P | 100 tests | $278.00 | + | |
8408 | 已糖激酶检测试剂盒 HK | 100 tests | $346.00 | + | |
8418 | 葡萄糖检测试剂盒 GL | 100 tests | $308.00 | + | |
8428 | 葡糖6磷酸脱氢酶检测试剂盒 G6PDH | 100 tests | $278.00 | + | |
8438 | 尿酸检测试剂盒 US | 100 tests | $405.00 | + | |
8448 | 铁检测试剂盒 | 100 tests | $331.00 | + | |
8458 | 黄嘌呤氧化酶检测试剂盒 XO | 100 tests | $150.00 | + | |
8458 | 黄嘌呤氧化酶检测试剂盒 XO | 100 tests | $150.00 | + | |
8468 | 甘油检测试剂盒 GLY | 100 tests | $241.00 | + | |
8478 | 丙氨酸转氨酶检测试剂盒 ALT | 100 tests | $322.00 | + | |
8498 | 甘油三酸酯检测试剂盒 TG | 100 tests | $309.00 | + | |
8508 | 3-甘油磷酸脱氢酶检测试剂盒 GPDH | 100 tests | $458.00 | + | |
8518 | 总谷胱甘肽检测试剂盒 TGA | 100 tests | $280.00 | + | |
8518 | 总谷胱甘肽检测试剂盒 TGA | 100 tests | $280.00 | + |