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Human Intestinal Microvascular Endothelial Cell Lysate (HIMEC Lysate) is prepared from early passage Human Intestinal Microvascular Endothelial Cells using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF). Lysate concentration is determined by Beckman Coulter DU 800 spectrophotometer. Lysate is diluted and stored in Storage Buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% ?-mercaptoethanol). Lysate from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.
货号 | 2906 |
产地 | 美国 |
缩写 | HIMEC Lysate |
规格 | 200μg |
用途 | 科研 |
储存 | 液氮 |
运输 | 干冰 |
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细胞裂解物
细胞裂解物
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