Liver Mononuclear Cells (LMC) are located in the liver and perform vital functions for the innate and adaptive immune system. Primary LMC are a heterogeneous population of small lymphocytes, large granular lymphocytes, monocytes, and granulocytes. Primary human LMC (HLMC) can be utilized to study the innate and adaptive immune system. HLMC from ScienCell Research Laboratories are isolated from human liver. HLMC are depleted of erythrocytes and hepatic macrophages, cryopreserved immediately after isolation, and delivered frozen. HLMC are a mixed population of cells that include T-lymphocytes, B-lymphocytes, NK-cells, and monocytes. Each vial contains at least 10 million cells in 1 ml volume. HLMC are quality control tested for viability. HLMC are negative for are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLMC can be maintained for a short period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture. Recommended Medium It is recommended to use HematoGro Medium (HeGM, Cat. #5501) for short-term maintenance of HLMC in vitro.