ScienCell's GeneQuery™ Human cDNA Evaluation Kit, Deluxe (GQH-CED) assesses cDNA quality. The kit verifies successful reverse transcription of messenger RNA (mRNA) to complementary DNA (cDNA), reveals the presence of genomic DNA (gDNA) contamination in cDNA samples, and detects qPCR inhibitor contamination. Good quality cDNA is a critical component for successful gene expression profiling. The GQH-CED kit is highly recommended for cDNA applications such as GeneQuery™ qPCR arrays.

Each primer set included in GQH-CED qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.

Quality Control

Each primer set is validated by qPCR melt curve and amplification curve analyses. The PCR products are analyzed by gel electrophoresis to confirm single band amplification.