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人成纤维细胞生长因子信号通路qPCR检测试剂盒
Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit
品牌:
ScienCell
货号:
GK040
价格:
$286
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ScienCell's GeneQuery? Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit (GQH-FGF) is designed to facilitate gene expression profiling of 88 key genes involved in Fibroblast Growth Factor (FGF) signaling pathways. FGFs are involved in a wide variety of biological functions such as embryonic development, organogenesis, angiogenesis, tissue regeneration and wound healing. Through their binding with FGF receptors (FGFRs), FGFs activate and regulate many downstream signaling pathways, such as MAPK, JNK and STAT/NF-κB. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Secreted FGFs
    • FGF1 superfamily: FGF1, FGF2
    • FGF4 superfamily: FGF4, FGF5
    • FGF7 superfamily: FGF3, FGF7, FGF10
    • FGF8 superfamily: FGF17, FGF18
    • FGF9 superfamily: FGF9, FGF16, FGF20
  • Endocrine FGFs: FGF19, FGF23
  • Intracellular FGFs: FGF13, FGF14
  • FGF receptors: FGFR1, FGFR2, FGFR3, FGFR5
  • FGF signaling inhibitors: IL17RD, SPRY2, SPRY4, PTPN1, DUSP1, DUSP6
  • Activated downstream pathways
    • MAPK pathway: FRS2, GAB1, SOS1, PTPN11, SHC1, RAS, BRAF, MAPK1, MAPK3, MAP2K1, MAP2K2
    • PI3K/AKT pathway: AKT1, AKT2, AKT3, PI3Ks, FOXO1, FOXO3, BAD, BAX, CASP9
    • JNK pathway: MAPK8, MAPK9, MAPK14, RAC1, ATF2, MAP2K4, MAP2K6
    • STAT/NF-κB pathway: STAT1, STAT3, NFKB1, NFKB2, REL, MMP9

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery? Human Fibroblast Growth Factor Signaling Pathway qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

DAG1

FGF16

FGF4

FOS

IL17RD

MAPK14

NFATC2

PIK3CD

PLCG1

SH2B1

β-actin

B

AKT2

DUSP1

FGF17

FGF5

FOXO1

JAK2

MAPK3

NFATC3

PIK3CG

PTPN1

SHC1

GAPDH

C

AKT3

DUSP6

FGF18

FGF7

FOXO3

KRAS

MAPK8

NFATC4

PIK3R1

PTPN11

SOS1

LDHA

D

ATF2

ELK1

FGF19

FGF9

FOXO4

MAP2K1

MAPK9

NFKB1

PIK3R2

RAC1

SPRY2

NONO

E

BAD

FGF1

FGF2

FGFR1

FRS2

MAP2K2

MARCKS

NFKB2

PIK3R3

RAF1

SPRY4

PPIH

F

BAX

FGF10

FGF20

FGFR2

GAB1

MAP2K4

MMP9

NRAS

PIK3R4

REL

SRC

GDC

G

BRAF

FGF13

FGF23

FGFR3

GRB2

MAP2K6

NFAT5

PIK3CA

PIK3R5

RELA

STAT1

PPC

H

CASP9

FGF14

FGF3

FGFR5

HRAS

MAPK1

NFATC1

PIK3CB

PIK3R6

RELB

STAT3

NTC

 

* gene selection may be updated based on new research and development

货号GK040
产地美国
缩写GQH-FGF
规格96Tests
用途科研
储存4度
运输冰袋
No Publication available at this time
There are no product questions yet. 
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