ScienCell's GeneQuery? Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction. Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation. Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B. A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal” and a “reverse signal.” Brief examples of how included genes may be grouped according to their function are shown below:
- Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4
- Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2
- Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1
- Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA
- Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN
GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery? Human Ephrin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTB |
AP2S1 |
DNM1 |
EFNB3 |
EPHB2 |
GRIA1 |
LIMK1 |
MYL6 |
PIK3CG |
RAP1A |
TF |
β-actin |
|
B |
ACTG1 |
APH1A |
EFNA1 |
EPHA1 |
EPHB3 |
GRIN1 |
LYN |
MYL9 |
PIK3R1 |
RASA1 |
TIAM1 |
GAPDH |
|
C |
ACTN1 |
CAV1 |
EFNA2 |
EPHA2 |
EPHB4 |
GRIN2B |
MAP2K1 |
NCK1 |
PSEN2 |
RGS3 |
TLN1 |
LDHA |
|
D |
ADAM10 |
CDC42 |
EFNA3 |
EPHA3 |
FN1 |
HRAS |
MAPK1 |
NCK2 |
PSENEN |
RHOA |
VAV2 |
NONO |
|
E |
AP2A1 |
CFL1 |
EFNA4 |
EPHA4 |
FYN |
ITGA5 |
MMP2 |
NCSTN |
PTK2 |
ROCK1 |
VAV3 |
PPIH |
|
F |
AP2A2 |
CLTA |
EFNA5 |
EPHA5 |
GNAI1 |
ITGAV |
MMP9 |
PAK1 |
PXN |
SDC2 |
VCL |
GDC |
|
G |
AP2B1 |
CLTB |
EFNB1 |
EPHA7 |
GRB2 |
ITGB1 |
MYH10 |
PAK2 |
RAC1 |
SRC |
VEGFA |
PPC |
|
H |
AP2M1 |
CLTC |
EFNB2 |
EPHB1 |
GRB7 |
KDR |
MYH9 |
PIK3CA |
RAF1 |
SYNJ1 |
WASL |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK089 |
| 产地 | 美国 |
| 缩写 | GQH-EPH |
| 规格 | 96Tests |
| 用途 | 科研 |
| 储存 | 4度 |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK091 | 人肝星状细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK092 | 人胰腺星状细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK093 | Human Renal Mesangial Cell Biology qPCR Array Kit | 96Tests | $286 | + | |
| GK094 | Human Mesenchymal Stem Cell Differentiation qPCR Array | 96tests | $286 | + | |
| GK094 | Human Mesenchymal Stem Cell Differentiation qPCR Array | 96Tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK096 | 人类雪旺细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK097 | 人类平滑肌细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK105 | 人类ECM降解qPCR检测试剂盒 | 96tests | $286 | + | |
| GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
