ScienCell's GeneQuery™ human cell cycle qPCR array kit (GQH-CCY) is designed to facilitate gene expression profiling of key genes involved in human cell cycle progression. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Cell Cycle qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ANAPC2 |
BUB3 |
CCND2 |
CDC20 |
CDK6 |
CHEK1 |
E2F1 |
HDAC1 |
MCM3 |
RAD1 |
SMAD2 |
β-actin |
|
B |
ATM |
CASP3 |
CCND3 |
CDC25A |
CDK7 |
CHEK2 |
E2F2 |
HDAC2 |
MCM4 |
RAD17 |
SMC3 |
GAPDH |
|
C |
ATR |
CCNA1 |
CCNE2 |
CDC25C |
CDK8 |
CKS1B |
E2F4 |
HUS1 |
MCM5 |
RAD51 |
STAG1 |
LDHA |
|
D |
AURKB |
CCNA2 |
CCNF |
CDC34 |
CDKN1A |
CKS2 |
E2F5 |
KNTC1 |
MDM2 |
RAD9A |
TFDP1 |
NONO |
|
E |
BCCIP |
CCNB1 |
CCNG1 |
CDC6 |
CDKN1B |
CUL1 |
EP300 |
KPNA2 |
MYC |
RB1 |
TFDP2 |
PPIH |
|
F |
BIRC5 |
CCNB2 |
CCNH |
CDK1 |
CDKN2A |
CUL2 |
GADD45A |
MAD2L1 |
NBN |
RBL1 |
TGFB1 |
GDC |
|
G |
BRCA1 |
CCNC |
CCNT1 |
CDK2 |
CDKN2B |
CUL3 |
GSK3B |
MAD2L2 |
ORC1 |
RBL2 |
TP53 |
PPC |
|
H |
BRCA2 |
CCND1 |
CDC16 |
CDK4 |
CDKN3 |
DBF4 |
GTSE1 |
MCM2 |
PCNA |
SKP2 |
WEE1 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK003 |
| 产地 | 美国 |
| 缩写 | GQH-CCY |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK101 | 人类Synoviocyte细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK102 | 人类滋养层细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK105 | 人类ECM降解qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK106 | 人类焦点粘附复合物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK107 | 人内皮素信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK111 | 人凝集素调节免疫应答qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK113 | 人类神经干细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK114 | 人类小胶质细胞极化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK115 | 人类骨髓谱系标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK116 | 人B细胞和T细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK117 | 人胸腺细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK118 | 人类先天淋巴细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK119 | 人类神经祖细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK121 | 人PD-1 / PD-L1检查点途径qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK123 | 人类生长因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK124 | 人类转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK125 | 人干细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK126 | 人类细胞表面标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
