ScienCell's GeneQuery™ Human Circadian Rhythm qPCR Array Kit (GQH-CIR) profiles 88 key genes involved in the regulating changes that occur on a roughly 24-hr cycle. Circadian rhythms are primarily responses to changes in light and are found in most living organisms. Not to be confused with biological clocks, circadian rhythms can influence sleep patterns, hormone release, body temperature, and other homeostatic functions. Below are brief examples of how included genes may be grouped according to their function:
- Clock regulation: ARNTL, BHLHE40, CLOCK, PER1, RORA
- Casein kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1
- CREB pathway: CAMK2A, HTR7, MAPK1, PRKACB, AANAT
- Transcription factors: SMAD4, IRF1, NFIL3, RORC, SP1,
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Circadian Rhythm qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
AANAT |
BHLHE41 |
CREB3 |
CSNK2A1 |
HDAC1 |
KLF9 |
MYOD1 |
NR2F6 |
PRKAB1 |
PRKCA |
RORC |
β-actin |
|
B |
ADORA1 |
BTRC |
CREBBP |
CUL1 |
HDAC2 |
KMT2A |
NCOA2 |
PER1 |
PRKAB2 |
PRKCB |
SIN3A |
GAPDH |
|
C |
ADORA2A |
CAMK2A |
CRX |
DBP |
HEBP1 |
LGR4 |
NFIL3 |
PER2 |
PRKACB |
PROK2 |
SKP1 |
LDHA |
|
D |
AHR |
CAMK2B |
CRY1 |
EGR1 |
HTR7 |
MAPK1 |
NGFR |
PER3 |
PRKACG |
PROX1 |
SMAD4 |
NONO |
|
E |
ARNTL |
CDK5R1 |
CRY2 |
EGR3 |
ID1 |
MAPK14 |
NOCT |
PPARA |
PRKAG2 |
RBX1 |
SP1 |
PPIH |
|
F |
ARNTL2 |
CHEK1 |
CSNK1A1 |
EPO |
ID2 |
MAPK3 |
NONO |
PPARGC1A |
PRKAG3 |
ROCK2 |
SREBF1 |
GDC |
|
G |
ATR |
CLOCK |
CSNK1D |
FBXL3 |
IRF1 |
MMAB |
NPAS2 |
PRKAA1 |
PRKAR1A |
RORA |
STAT5A |
PPC |
|
H |
BHLHE40 |
CREB1 |
CSNK1E |
FBXW11 |
KLF10 |
MTNR1B |
NR1D2 |
PRKAA2 |
PRKAR2A |
RORB |
UBE3A |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK060 |
| 产地 | 美国 |
| 缩写 | GQH-CIR |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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