ScienCell's GeneQuery™ Human Microglia Cell Biology qPCR Array (GQH-MGL) facilitates gene expression profiling of 88 key genes involved in microglial regulation and function. Microglia are considered to be the main cells of defense in the central nervous system (CNS). Though often touted as “the resident macrophages of the CNS,” microglia distinguish themselves from macrophages in regulation and through specialized roles. Examples of how included genes may be grouped according to their function are shown below:
- Classical priming: BIRC3, IFNG, IL12A, CXCL10, TYMP
- Alternative priming: GAS7, IGF1, CXCL2, SPP1, MRC1
- Microglia markers: PTPRC, ITGAX, AIF1, CD40, EDA
- Sensome: GPR34, CXCR2, ADORA3, LTF, SIGLECH
- Receptors: MARCO, MSR1, P2RX4, SIRPA, CSF1R
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Microglia Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ADORA3 |
CD40 |
CX3CR1 |
F13A1 |
HLA-DQB1 |
IRF1 |
MARCO |
NOS2 |
PTGES |
SLCO2B1 |
TMEM8C |
β-actin |
|
B |
AIF1 |
CD47 |
CXCL10 |
FGL2 |
HLA-DRA |
IRF8 |
MMP12 |
P2RX4 |
PTGS2 |
SPI1 |
TNF |
GAPDH |
|
C |
BIRC3 |
CD68 |
CXCL2 |
GAS7 |
HLA-DRB1 |
ITGAM |
MRC1 |
P2RX7 |
PTPRC |
SPP1 |
TREM2 |
LDHA |
|
D |
CASP1 |
CD80 |
CXCL9 |
GPR34 |
IFNG |
ITGAX |
MSR1 |
P2RY1 |
PYCARD |
TGFB1 |
TXNIP |
NONO |
|
E |
CCL8 |
CD86 |
CXCR2 |
GPR84 |
IGF1 |
ITGB5 |
MYB |
P2RY12 |
RUNX1 |
TLR2 |
TYMP |
PPIH |
|
F |
CD163 |
CLEC5A |
ECSCR |
HAVCR2 |
IL12A |
LGALS3 |
MYD88 |
P2RY2 |
SIGLEC5 |
TLR3 |
TYROBP |
GDC |
|
G |
CD200R1 |
CLEC7A |
EDA |
HEXB |
IL1B |
LILRA5 |
NLRC4 |
PANX1 |
SIRPA |
TLR4 |
UPK1B |
PPC |
|
H |
CD33 |
CSF1R |
EGR2 |
HLA-DQA1 |
IL6 |
LTF |
NLRP3 |
PILRA |
SLAMF9 |
TLR5 |
XIAP |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK069 |
| 产地 | 美国 |
| 缩写 | GQH-MGL |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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