ScienCell's GeneQuery™ Human Axon Guidance qPCR Array (GQH-AXN) profiles 88 key genes involved in neural pathfinding. During neural development, neurons send projections otherwise known as axons to reach targets and transmit electric signals. Axons follow specific paths and the underlying pathfinding mechanism is an area of growing research. Below are brief examples of how included genes may be grouped according to their signaling pathways or protein classes:
- Ephrins: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1
- Semaphorins: SEMA3A, SEMA4D, SEMA5A, SEMA6B, SEMA7A
- WNT signaling: WNT4, WNT5A, SHH, SMO, FZD3
- Netrin signaling: NTN1, NTN4, NTNG1, NTNG2, DCC, UNC5A
- Cell adhesion molecules: CAMK2A, CAMK2B, CAMK2D, CAMK2G
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Axon Guidance qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABL1 |
CAMK2B |
EFNA1 |
EPHA1 |
EPHB3 |
NRP1 |
RAC1 |
SEMA3D |
SEMA4F |
SLIT2 |
TRPC6 |
β-actin |
|
B |
ABLIM3 |
CAMK2D |
EFNA2 |
EPHA2 |
EPHB4 |
NRP2 |
RAF1 |
SEMA3E |
SEMA4G |
SLIT3 |
UNC5A |
GAPDH |
|
C |
ARHGEF12 |
CAMK2G |
EFNA3 |
EPHA3 |
F2RL2 |
NTN1 |
ROBO1 |
SEMA3F |
SEMA5A |
SMO |
UNC5B |
LDHA |
|
D |
BMP7 |
CFL1 |
EFNA4 |
EPHA4 |
FES |
NTN4 |
ROBO2 |
SEMA3G |
SEMA6B |
TRPC1 |
UNC5C |
NONO |
|
E |
BMPR1B |
CFL2 |
EFNA5 |
EPHA5 |
FYN |
NTNG1 |
ROCK1 |
SEMA4A |
SEMA6D |
TRPC1 |
UNC5D |
PPIH |
|
F |
BMPR2 |
CXCR4 |
EFNB1 |
EPHA7 |
FZD3 |
NTNG2 |
RYK |
SEMA4B |
SEMA7A |
TRPC3 |
WNT4 |
GDC |
|
G |
BOC |
DCC |
EFNB2 |
EPHB1 |
NCK1 |
PAK2 |
SEMA3A |
SEMA4C |
SHH |
TRPC4 |
WNT5A |
PPC |
|
H |
CAMK2A |
DPYSL2 |
EFNB3 |
EPHB2 |
NFATC2 |
PAK4 |
SEMA3C |
SEMA4D |
SLIT1 |
TRPC5 |
WNT5B |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK084 |
| 产地 | 美国 |
| 缩写 | GQH-AXN |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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