Mesenchymal Stem Cell Medium-animal component free (MSCM-acf), when used with Mesenchymal Stem Cell Growth Supplement-animal component free (MSCGS-acf, Cat #7572), is a chemically defined medium designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is xeno-free and does not contain any animal- or human-origin materials. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mesenchymal stem cells in vitro. 

Note: MSCGS-acf and P/S are not pre-mixed in MSCM-acf; they must be added separately to make the complete MSCM-acf.