Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.
ScienCell's Absolute Pig Telomere Length Quantification qPCR Assay Kit (APTLQ) is designed to directly measure the average telomere length of a pig cell population. The telomere primer set recognizes and amplifies telomere sequences. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on pig chromosome 13, and serves as reference for data normalization. The reference genomic DNA sample with known telomere length serves as a reference for calculating the telomere length of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.
货号 | P8918 |
产地 | 美国 |
缩写 | APTLQ |
规格 | 100 reactions |
用途 | 科研 |
储存 | -20度 |
运输 | 干冰 |
Has your paper been published using one or more of Sciencell products? Get rewarded! Receive 10% off one order*. See details >>
1.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.
2.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.
3.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197
4.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184
5.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40
货号 | 产品名称 | 规格 | 价格 | 指令 | |
8258 | 碱性磷酸酶活力检测试剂盒 ALP | 500 tests | $396 | + | |
8268 | 线粒体分离试剂盒 MITOISO | 100 tests | $334 | + | |
8278 | 细胞色素C氧化酶检测试剂盒 COX | 100 tests | $361 | + | |
8278 | 细胞色素C氧化酶检测试剂盒 COX | 100 tests | $361 | + | |
8288 | 碱性磷酸酶染色试剂盒(红) ALPr | 500 tests | $450 | + | |
8298 | 人间充质干细胞脂肪形成检测试剂盒 HMSC-A PCR | 50 reactions | $324 | + | |
8308 | L-乳酸盐检测试剂盒 LAC | 100 tests | $215 | + | |
8318 | 柠檬酸合成酶检测试剂盒 CS | 50 tests in cuvette or 250 tests in 96-well plate | $477 | + | |
8318 | 柠檬酸合成酶检测试剂盒 CS | 50 tests in cuvette or 250 tests in 96-well plate | $477 | + | |
8328 | 人间充质干细胞软骨形成检测试剂盒 HMSC-C PCR | 50 reactions | $324 | + | |
8338 | 谷氨酸检测试剂盒 GLU | 100 tests | $415 | + | |
8358 | 谷氨酸脱氢酶检测试剂盒 GDH | 100 tests | $415 | + | |
8368 | NAD/NADH检测试剂盒 NAD | 100 tests | $418 | + | |
8368 | NAD/NADH检测试剂盒 NAD | 100 tests | $418 | + | |
8378 | 阿尔新蓝染色试剂盒 ABlue | 100 tests | $150 | + | |
8388 | 储液瓶 传统风格 125ml 45mm颈尺寸 带盖 灭菌 | 100 tests | $354 | + | |
8398 | 葡萄糖6磷酸酶检测试剂盒 G6P | 100 tests | $267 | + | |
8408 | 已糖激酶检测试剂盒 HK | 100 tests | $332 | + | |
8418 | 葡萄糖检测试剂盒 GL | 100 tests | $296 | + | |
8428 | 葡糖6磷酸脱氢酶检测试剂盒 G6PDH | 100 tests | $267 | + | |
8438 | 尿酸检测试剂盒 US | 100 tests | $389 | + | |
8448 | 铁检测试剂盒 | 100 tests | $318 | + | |
8458 | 黄嘌呤氧化酶检测试剂盒 XO | 100 tests | $144 | + | |
8458 | 黄嘌呤氧化酶检测试剂盒 XO | 100 tests | $144 | + | |
8468 | 甘油检测试剂盒 GLY | 100 tests | $231 | + | |
8478 | 丙氨酸转氨酶检测试剂盒 ALT | 100 tests | $309 | + | |
8498 | 甘油三酸酯检测试剂盒 TG | 100 tests | $297 | + | |
8508 | 3-甘油磷酸脱氢酶检测试剂盒 GPDH | 100 tests | $440 | + | |
8518 | 总谷胱甘肽检测试剂盒 TGA | 100 tests | $269 | + | |
8518 | 总谷胱甘肽检测试剂盒 TGA | 100 tests | $269 | + |