Lymphatic Mononuclear Cells (LyMC) are located in the lymph nodes and perform vital functions for the innate and adaptive immune system. Primary LyMC are a mixed population of single nucleus cells which include T-cells (helper T-cells, cytotoxic T-cells, gamma delta T-cells), Bcells, and natural killer (NK) cells. Primary human LyMC (HLyMC) can be used to study the innate and adaptive immune system. HLyMC from ScienCell Research Laboratories are isolated from human lymph nodes. HLyMC are depleted of erythrocytes, cryopreserved immediately after isolation, and delivered frozen. HLyMC are a mixed population of cells that include T-cells, B-cells, and NK cells. Each vial contains at least 10 million cells in 1 ml volume. HLyMC are quality control tested for viability. HLyMC are negative for mycoplasma, bacteria, yeast, and fungi. HLyMC can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture. Recommended Medium It is recommended to use HematoGro Medium (HeGM, Cat. #5501) for short-term maintenance of HLyMC in vitro.