The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Neurons are dynamically polarized cells responsible for electrochemically transmitting information throughout the nervous system. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. In rat, the adult brain has been shown to contain 200 million neurons. The dorsal spinal cord relays sensory information from the body to the brain. Sensory nerves converge and enter the spinal cord on the dorsal side, where the information then ascends to the brain via white matter tracts. Injuries to the dorsal spinal cord disrupt pain, temperature, and proprioceptive sensation. Primary rat neurons-dorsal spinal cord (RN-dsc) can be used to study the mechanisms involved in spinal cord injuries. RN-dsc from ScienCell Research Laboratories are isolated from the embryonic day 14 CD® IGS rat spinal cord. RN-dsc were cryopreserved at P0 and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. RN-dsc are characterized by immunofluorescence with antibodies specific to β-tubulin III. RN-dsc are negative for mycoplasma, bacteria, yeast, and fungi. RN-dsc are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RN-dsc are not recommended for expanding or long-term cultures since the cells do not proliferate in culture. Recommended Medium It is recommended to use neuronal medium (NM, Cat. #1521) for culturing RN-dsc in vitro.