The salivary gland produces enzyme-containing saliva to lubricate the oral cavity and assist with digestion. It consists of three pairs of major salivary glands and many minor ones in the oral cavity. Histology studies show that the salivary gland is a compound tubuloalveolar gland, surrounded by connective tissue to further divide the gland into lobes and lobules. The secretory end pieces consist of a spherical mass of cells called acinus. Rat salivary gland fibroblasts (RSGF) are embryonic mesoderm derived mesenchymal cells and they are responsible for synthesis of extracellular matrix such as type I and/or type III collagen. RSGF are very versatile and provide a useful tool for studying collagenous fibroma and oral neoplasms. RSGF from ScienCell Research Laboratories are isolated from postnatal day 2 rat salivary gland. RSGF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RSGF characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. RSGF are negative for mycoplasma, bacteria, yeast, and fungi. RSGF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RSGF in vitro.