The liver is critical for many vital functions including: energy metabolism, biotransformation of xenobiotics, synthesis of plasma proteins under physiological and pathophysiological conditions, and detoxification of substances [1]. Human Hepatocytes (HH) are multifunctional cells that produce proteins required for protein synthesis, synthesize cholesterol, store proteins, produce and secrete bile, and detoxify substances [1-3]. Cultured HH are an excellent in vitro model for studying liver function, metabolism, and liver disease. Understanding the molecular mechanisms of the liver may help to elucidate new therapies for treatment of hepatic disease. HH from ScienCell Research Laboratories are isolated from human liver. HH are cryopreserved immediately after purification and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HH are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or albumin. HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HH are not recommended for expanding or long term cultures since the cells do not proliferate in culture. Recommended medium: It is recommended to use Hepatocyte Medium (HM, Cat. #5201) for the culturing of HH in vitro.