Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. The developmental plasticity of MSC has generated tremendous interest because of the potential use of mesenchymal stem cells in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs. DMSC-ad from ScienCell Research Laboratories are isolated from dog adipose tissue. DMSC-ad are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. DMSC-ad are characterized by immunofluorescence with antibodies specific to CD90 and Oil Red O staining after adipogenic differentiation. DMSC-ad are negative for mycoplasma, bacteria, yeast and fungi. DMSC-ad are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing DMSC-ad in vitro.