Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs. HMSC-ad from ScienCell Research Laboratories are isolated from human adipose tissue. HMSC-ad are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMSC-ad are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HMSC-ad are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-ad are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HMSC-ad in vitro.