Fibroblasts are mesenchymal cells which perform many vital functions during development and in adulthood. They are responsible for much of the synthesis of extracellular matrix in connective tissue and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types. Human oral fibroblasts (HOrF), located in the oral cavity, have the ability to rapidly repair defects in the oral cavity. HOrF, in contrast to skin fibroblasts, can more quickly reorganize the extracellular matrix and migrate for wound repair. Abnormal proliferation of HOrF can lead to the development of oral squamous cell carcinoma. HOrF are a useful model for elucidating the mechanisms of fibrosis and developing treatments for oral cancers. HOrF from ScienCell Research Laboratories are isolated from human oral tissue. HOrF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HOrF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HOrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOrF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HOrF in vitro.