The cervix is the lower portion of the uterus and functions to promote fertility and protect the female reproductive tract and fetus from potential pathogens. In addition, the cervix plays a critical role in childbirth by effacing and dilating to allow the baby to pass out of the uterus. Cervical epithelial cells (CerEpiC), which line the cervix, maintain structural integrity and limit the passage of molecular and cellular substances into the cervix using intercellular junctions. Studies have shown that patients infected with human papillomavirus (HPV) causes inflammation of the epithelial cell layer which can lead to the development of cervical carcinoma. Primary human CerEpiC (HCerEpiC) are a useful in vitro model for studying the pathophysiology of cervical polyps, HPV, and cervical cancer. HCerEpiC from ScienCell Research Laboratories are isolated from human uterus. HCerEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCerEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HCerEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCerEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Cervical Epithelial Cell Medium (CerEpiCM, Cat. #7061) for culturing HCerEpiC in vitro.