ScienCell's GeneQuery? human neural transmission and membrane trafficking qPCR array kit (GQH-NTM) is designed to facilitate gene expression profiling of key genes involved in (i) human neurotransmission; (ii) membrance trafficking; and (iii) related diseases such as generalized anxiety disorder, DDC deficiency, TH deficiency, and SPR deficiency. 88 genes are selected in this kit based on database and literature research.
GeneQuery? PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery? Human Neural Transmission and Membrane Trafficking qPCR Array Plate Layout* (8 controls in Bold and Italic)
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ADRA1A |
BDNF |
CHRNA5 |
DRD2 |
GABRA2 |
GABRG3 |
GRIK1 |
GRM3 |
HTR2A |
OPTN |
SNAP25 |
β-actin |
|
B |
ADRA1D |
BTD |
CHRNA6 |
DRD4 |
GABRA4 |
GABRQ |
GRIK2 |
GRM4 |
HTR2C |
OTC |
SOD1 |
GAPDH |
|
C |
ADRA2A |
CHMP2B |
CHRNA7 |
DRD5 |
GABRA5 |
GABRR2 |
GRIK4 |
GRM6 |
HTR3A |
PTS |
SPR |
LDHA |
|
D |
ADRB2 |
CHRM1 |
CHRNE |
FIG4 |
GABRB1 |
GCH1 |
GRIK5 |
GRM7 |
HTR4 |
QDPR |
TARDBP |
NONO |
|
E |
ADRB3 |
CHRM4 |
COMT |
FUS |
GABRB3 |
GH1 |
GRIN2A |
HTR1A |
HTR7 |
RAB10 |
TH |
PPIH |
|
F |
AKR1B1 |
CHRM5 |
DBH |
G6PD |
GABRE |
GRIA1 |
GRIN2B |
HTR1B |
LLGL1 |
SLC6A2 |
TPH1 |
GDC |
|
G |
ALDH5A1 |
CHRNA3 |
DHFR |
GABBR1 |
GABRG1 |
GRIA2 |
GRIN2C |
HTR1D |
LPL |
SLC6A3 |
VAPB |
PPC |
|
H |
ALS2 |
CHRNA4 |
DRD1 |
GABBR2 |
GABRG2 |
GRIA3 |
GRM1 |
HTR1F |
MAOA |
SLC6A4 |
VCP |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK008 |
| 产地 | 美国 |
| 缩写 | GQH-NTM |
| 规格 | 96Tests |
| 用途 | 科研 |
| 储存 | 4° |
| 运输 | 冰袋 |
