ScienCell's GeneQuery? Human Renal Mesangial Cell Biology qPCR Array Kit (GQH-RMB) is designed to facilitate gene expression profiling of 88 key genes involved in essential mesangial cell biology and functions involved in glomerular physiology and pathophysiology. Brief examples of how included genes may be grouped according to their functions are shown below:
- Renal mesangial cell markers: ACTA1, FN1, PDGFRB, TNS1, ITGA8
- Mesangial matrix assembly: SERPINB7, Collagen type 4/5, Laminin α/β/γ, SDC2, HSPG2, CSPG5, NID1, ITGA8
- Growth factor modulation: IGF1, PTN, MFGE8, GDF10, CTGF, NGF, PSEN2, SH3KBP1, ADAM12, AFAP1L2, SHC3
- Vasculogenesis: VCAM1, ANGPT2, HOPX, MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF
- Endocytosis: CLTA, CLTB, CLTC, AP2A1, AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9, TGFB receptors
- Transmembrane transporter activity: KCNMB1, GJA5, CACNA2D1, ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2
- Genes implicated in
- Mesangial proliferative glomerulonephritis: SERPINB7, IL6, CD79A, CCL2, FGF2, EDN1, CCR5, GAS6, AXL
- Diffuse mesangial sclerosis: ACTN4, TRPC6, INF2, WT1, PLCE1, PAX2, ARHGDIA, LAMB2, LAMC1
GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery? Human Renal Mesangial Cell Biology qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ABCC9 |
AP2A1 |
CCL2 |
COL5A1 |
FHL2 |
IGF1 |
LAMA2 |
MFGE8 |
PDGFRB |
SFRP2 |
TGFBR3 |
β-actin |
|
B |
ACTA1 |
AP2A2 |
CCR5 |
COL5A2 |
FN1 |
IL6 |
LAMA3 |
MYH11 |
PIEZO2 |
SH3KBP1 |
TNS1 |
GAPDH |
|
C |
ACTN4 |
AP2B1 |
CD79A |
COL5A3 |
GAS6 |
INF2 |
LAMA4 |
NGF |
PLCE1 |
SHC3 |
TRPC1 |
LDHA |
|
D |
ADAM12 |
AP2M1 |
CLTA |
CSPG5 |
GDF10 |
ITGA8 |
LAMA5 |
NID1 |
PLN |
SMAD1 |
TRPC4 |
NONO |
|
E |
ADAMTS1 |
AP2S1 |
CLTB |
CTGF |
GJA5 |
KCNMB1 |
LAMB1 |
NID2 |
PSEN2 |
SMAD2 |
TRPC6 |
PPIH |
|
F |
AFAP1L2 |
ARHGDIA |
CLTC |
EDN1 |
HDAC9 |
KCNQ5 |
LAMB2 |
NRP2 |
PTN |
SMAD3 |
VCAM1 |
GDC |
|
G |
AGTR1 |
AXL |
COL4A1 |
ETS1 |
HOPX |
KCNRG |
LAMC1 |
P2RX1 |
SDC2 |
TGFBR1 |
WT1 |
PPC |
|
H |
ANGPT2 |
CACNA2D1 |
COL4A2 |
FGF2 |
HSPG2 |
LAMA1 |
MECOM |
PAX2 |
SERPINB7 |
TGFBR2 |
ZFYVE9 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK093 |
| 产地 | 美国 |
| 缩写 | GQH-RMB |
| 规格 | 96Tests |
| 用途 | 科研 |
| 储存 | 4度 |
| 运输 | 胶冰 |
