ScienCell's GeneQuery? Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:?????
- EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD
- EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1
- Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1
- Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B
- Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2
- Signaling pathways involved in MET
- Estrogen receptor: CAV2, ESR1, KRT19, TGFB3
- GPCR: AKT1, FZD7, GNG11, RAC1, RGS2
- PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5
GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.?
GeneQuery? Human Mesenchymal To Epithelial Transition qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
? |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACTA2 |
CAV2 |
COL3A1 |
F11R |
IGFBP4 |
MUC1 |
PIK3CG |
RGS2 |
SNAI3 |
TGFB2 |
VCAN |
β-actin |
|
B |
AKT1 |
CDH1 |
COL5A2 |
FGFBP1 |
ILK |
NODAL |
PIK3R1 |
RPS27A |
SOX10 |
TGFB3 |
VIM |
GAPDH |
|
C |
AKT2 |
CDH11 |
CTNNB1 |
FN1 |
JAG1 |
NOTCH1 |
PIK3R3 |
S100A4 |
SPINT2 |
TGFBR1 |
WNT11 |
LDHA |
|
D |
ARHGEF18 |
CDH2 |
DSP |
FOXC2 |
KRT14 |
PAX2 |
PIK3R5 |
SDC1 |
STAT3 |
TGFBR2 |
WNT5A |
NONO |
|
E |
AXL |
CDKN2A |
EGFR |
FZD7 |
KRT19 |
PDGFD |
PRKCZ |
SMAD2 |
STK11 |
TIMP1 |
WNT5B |
PPIH |
|
F |
BMP1 |
CDKN2B |
ERBB3 |
GNG11 |
LEF1 |
PDGFRB |
PRRX1 |
SMURF1 |
TCF3 |
TRIM28 |
WT1 |
GDC |
|
G |
BMP2 |
CLDN4 |
ESR1 |
GSC |
MMP2 |
PIK3CA |
RAB25 |
SNAI1 |
TCF4 |
TWIST1 |
ZEB1 |
PPC |
|
H |
BMP7 |
CLDN7 |
ETS1 |
HNRNPAB |
MST1R |
PIK3CD |
RAC1 |
SNAI2 |
TGFB1 |
UBA52 |
ZEB2 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK095 |
| 产地 | 美国 |
| 缩写 | GQH-MET |
| 规格 | 96tests |
| 用途 | 科研 |
| 储存 | 4度 |
| 运输 | 胶冰 |
