ScienCell's GeneQuery™ Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:
- Wound healing stages
- Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A
- Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG
- Proliferation and tissue remodeling: EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42
- Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1
- Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN
- Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6
- Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Skin Wound Healing qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ACTA2 |
COL1A2 |
CSK |
CXCL8 |
FGF2 |
IL10 |
ITGB1 |
MMP7 |
PDGFRB |
RAC1 |
TGFBR3 |
β-actin |
B |
ACTC1 |
COL3A1 |
CTGF |
EGF |
FGF7 |
IL1A |
ITGB3 |
MMP9 |
PLAT |
RHOA |
TIMP1 |
GAPDH |
C |
ANGPT1 |
COL4A1 |
CTNNB1 |
EGFR |
FIGF |
IL1B |
ITGB5 |
NGF |
PLAU |
SERPINE1 |
TNF |
LDHA |
D |
CCL2 |
COL4A3 |
CTSG |
ELANE |
HBEGF |
IL4 |
ITGB6 |
PDGFA |
PLAUR |
STAT3 |
VEGFA |
NONO |
E |
CD40LG |
COL5A1 |
CTSK |
F13A1 |
HGF |
IL6 |
MAPK1 |
PDGFB |
PLG |
TAGLN |
VEGFB |
PPIH |
F |
CDC42 |
COL5A3 |
CTSV |
F3 |
HIF1A |
IL6ST |
MAPK3 |
PDGFC |
PRTN3 |
TGFA |
VEGFC |
GDC |
G |
CDH1 |
CSF2 |
CXCL1 |
FGA |
IFNG |
ITGA1 |
MMP1 |
PDGFD |
PTEN |
TGFB1 |
WISP1 |
PPC |
H |
COL14A1 |
CSF3 |
CXCL2 |
FGF10 |
IGF1 |
ITGA5 |
MMP2 |
PDGFRA |
PTGS2 |
TGFB2 |
WNT5A |
NTC |
* gene selection may be updated based on new research and development
货号 | GK041 |
产地 | 美国 |
缩写 | GQH-SWH |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |
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