ScienCell's GeneQuery™ Human DNA Damage Sensing qPCR Array Kit (GQH-DDS) is designed to facilitate gene expression profiling of 40 key genes involved in DNA damage sensing. DNA damages, such as mutations, breaks, cross-links, deletions/insertions and translocations, are constantly generated due to internal and external stresses. Appropriate DNA damage responses must be implemented to maintain genome stability. Brief examples of how included genes may be grouped are shown below:
- DNA damage sensors: ATM, ATR, PRKDC, PARP1
- Cell cycle regulation: CHEK1, CHEK2, CCNB1, CCNB2, CCNB3, CCND1, CCND2, CCND3, CCNE2, CDK2, CDK4, CDK6
- ATM signaling pathway: ABL1, BRCA1, CDC25A, CDKN1A, CHEK1, CHEK2, GADD45A, RAD51, RBBP8, TP53
- ATR signaling pathway: CDC25A, CDC25C, CDK2, CHEK1, CHEK2, HUS1, MDM2, RAD1, RAD17, RAD9A, TP53
- Apoptosis: ABL1, ATM, BAX, BRCA1, CASP3, CASP9, CDKN1A, CHEK2, PRKDC, RAD91, TP53
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human DNA Damage Sensing qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ABL1 |
CCNB1 |
CDC25C |
FAS |
PRKDC |
β-actin |
ABL1 |
CCNB1 |
CDC25C |
FAS |
PRKDC |
β-actin |
|
B |
ATM |
CCNB2 |
CDK2 |
GADD45A |
RAD1 |
GAPDH |
ATM |
CCNB2 |
CDK2 |
GADD45A |
RAD1 |
GAPDH |
|
C |
ATR |
CCNB3 |
CDK4 |
GADD45B |
RAD17 |
LDHA |
ATR |
CCNB3 |
CDK4 |
GADD45B |
RAD17 |
LDHA |
|
D |
BAX |
CCND1 |
CDK6 |
HUS1 |
RAD51 |
NONO |
BAX |
CCND1 |
CDK6 |
HUS1 |
RAD51 |
NONO |
|
E |
BID |
CCND2 |
CDKN1A |
MDM2 |
RAD9A |
PPIH |
BID |
CCND2 |
CDKN1A |
MDM2 |
RAD9A |
PPIH |
|
F |
BRCA1 |
CCND3 |
CDKN1B |
MYC |
RB1 |
GDC |
BRCA1 |
CCND3 |
CDKN1B |
MYC |
RB1 |
GDC |
|
G |
CASP3 |
CCNE2 |
CHEK1 |
NBN |
RBBP8 |
PPC |
CASP3 |
CCNE2 |
CHEK1 |
NBN |
RBBP8 |
PPC |
|
H |
CASP9 |
CDC25A |
CHEK2 |
PARP1 |
TP53 |
NTC |
CASP9 |
CDC25A |
CHEK2 |
PARP1 |
TP53 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK061 |
| 产地 | 美国 |
| 缩写 | GQH-DDS |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK040 | 人成纤维细胞生长因子信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK041 | 人体皮肤伤口愈合qPCR检测套件 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK056 | 人体止血qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK060 | 人类昼夜节律qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK061 | 人类DNA损伤感应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK062 | 人类细胞生长和分裂qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK063 | 人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK069 | 人类小胶质细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK072 | 人类适应性免疫反应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK073 | 人类小胶质细胞Sensome qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK074 | 人角质细胞生物学qPCR检测 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK078 | 人类神经细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK079 | 人类软骨细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK080 | 人成骨分化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK081 | 人类周细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK084 | 人体轴突导向qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK087 | 人类染色质组织和重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK088 | 人VEGF信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK089 | 人类Ephrin信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK090 | 人体细胞连接qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK091 | 人肝星状细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK092 | 人胰腺星状细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK093 | Human Renal Mesangial Cell Biology qPCR Array Kit | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK094 | Human Mesenchymal Stem Cell Differentiation qPCR Array | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK096 | 人类雪旺细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK097 | 人类平滑肌细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK098 | 人类心脏肌细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK099 | 人类骨骼肌细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK100 | 人角质形成细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
