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人类Wnt信号靶基因qPCR检测试剂盒
Human Wnt Signaling Target Genes qPCR Array Kit
品牌:
ScienCell
货号:
GK028
价格:
$286
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ScienCell's GeneQuery™ Human Wnt Signaling Target Genes qPCR Array Kit (GQH-WTG) is designed to facilitate gene expression profiling of 88 key genes responsive to Wnt signaling activation and genes downstream of Wnt signaling induced transcription.  This kit includes genes regulated by both canonical and noncanonical Wnt signaling pathways.  Brief examples of how included downstream genes may be grouped according to their respective functions are shown below:

  • Transcription factors: HNF1A, LEF1, TCF7L1, TCF7L2, FOSL1
  • Cell cycling/growth: CCND1, CDKN2A, BIRC5, APC, TERC
  • Motility: S100A4, S100A6, NRCAM, KRT1, FN1
  • Angiogenesis: EDN1, PLAU, VEGFA, NOTCH1, EDNRA
  • Cancer misregulation: TWIST1, RET, JUN, NEDD9, MLLT10

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Wnt Signaling Target Genes qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCB1

CCND1

DOT1L

EP300

HES1

L1CAM

MSL1

NOTCH2

PRL

SOX2

TNFRSF19

β-actin

B

AMER1

CD44

EDN1

FGF18

HNF1A

LEF1

MYC

NRCAM

PTGS2

SOX9

TWIST1

GAPDH

C

APC

CDH1

EDN3

FGF20

ID2

MET

MYCN

PEX2

RET

SP5

TWIST2

LDHA

D

ATOH1

CDKN2A

EDNRA

FGF4

IGF1

MLLT10

NANOG

PITX2

RUNX2

TBC1D9

VCAN

NONO

E

BIRC5

CDX1

EGFR

FN1

IL6

MMP14

NANOS2

PLAU

S100A4

TCF7L1

VEGFA

PPIH

F

BMP4

CLDN1

EGR1

FOSL1

IRS1

MMP2

NDP

PLAUR

S100A6

TCF7L2

WISP1

GDC

G

CACNA1G

CTLA4

EGR2

GBX2

JUN

MMP7

NEDD9

POSTN

SOST

TERC

WISP2

PPC

H

CCNA2

CXCL8

EMP1

GJA1

KRT1

MMP9

NOTCH1

PPARD

SOX17

TIAM1

YAP1

NTC

 * gene selection may be updated based on new research and development

货号GK028
产地美国
缩写GQH-WTG
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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