ScienCell's GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:
- B cell receptor complex components: CD79A, CD79B
- Co-inhibitory receptors: CD22, CD72, FCGR2B
- Co-stimulators: CD81, CR2, IFITM1
- Expression adaptor proteins: BLNK, GAB2, GRB2, CARD11
- Kinases: LYN, SYK, PI3Ks
- Phosphatases: INPP5D, PTPN6
- MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
- PI3K/AKT pathway: PI3Ks, AKT1, AKT2
- IKK/NFKB pathway: NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AKT1 |
CD22 |
DAPP1 |
GSK3B |
INPP5D |
MAP2K1 |
MYC |
NFKBIE |
PIK3R3 |
PRKCD |
RELA |
β-actin |
|
B |
AKT2 |
CD72 |
ELK1 |
GTF2I |
INPPL1 |
MAP3K7 |
NCK1 |
NRAS |
PIP5K1A |
PTPN18 |
SH3BP2 |
GAPDH |
|
C |
ATF2 |
CD79A |
FCGR2B |
HCLS1 |
IRF4 |
MAP4K1 |
NFATC1 |
PDPK1 |
PIP5K1C |
PTPN6 |
SHC1 |
LDHA |
|
D |
BCL10 |
CD79B |
FOS |
HRAS |
JUN |
MAPK1 |
NFATC2 |
PIK3AP1 |
PLCG2 |
RAC1 |
SOS1 |
NONO |
|
E |
BLNK |
CD81 |
FYN |
IFITM1 |
KRAS |
MAPK3 |
NFATC3 |
PIK3CA |
PPP3CA |
RAC2 |
SOS2 |
PPIH |
|
F |
BTK |
CHUK |
GAB2 |
IKBKB |
LAT2 |
MAPK4 |
NFKB1 |
PIK3CB |
PPP3CB |
RAF1 |
SYK |
GDC |
|
G |
CARD11 |
CR2 |
GRB2 |
IKBKG |
LYN |
MAPK9 |
NFKBIA |
PIK3R1 |
PPP3R1 |
RASGRP3 |
TEC |
PPC |
|
H |
CBL |
DAG1 |
GSK3A |
ILF2 |
MALT1 |
MEF2D |
NFKBIB |
PIK3R2 |
PRKCB |
REL |
VAV2 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK020 |
| 产地 | 美国 |
| 缩写 | GQH-BRS |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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