ScienCell's GeneQuery™ Human Endothelin Signaling qPCR Array Kit (GQH-EDN) surveys 88 key genes involved in the endothelin signaling transduction with a particular focus on endothelin-1. Endothelins are secreted proteins that regulate vasoconstriction and are produced primarily in the endothelium. Endothelin receptors are also present in cells of the central nervous system and abnormal signaling may be involved in seizures and peripheral neuropathy. Other diseases implicated in aberrant endothelin signaling include cardiovascular disorders, vasospasm, cardiac hypertrophy, and Dengue fever. Brief examples of how included genes may be categorized are shown below:
- Calcium regulation: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D
- Cytoskeletal remodeling: CAPN2, MAP2K1, PLD1, PLD2, RHOA
- Cell cycling regulation: CCND3, CDC42, ITPR1, ITPR3, RIPK1
- Transcription factors: ELK1, FOS, JUN, MYC, SP1
- RAS superfamily GTPases: HRAS, KRAS, NRAS, RAC1, RAP1A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Endothelin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ADCY1 |
CACNA1D |
CDC42 |
EDNRA |
GSK3B |
MAP2K1 |
MYC |
PIK3R1 |
PPP1CA |
PTGS2 |
RIPK1 |
β-actin |
|
B |
ADCY3 |
CALM1 |
CGA |
EDNRB |
HBEGF |
MAP3K1 |
NOS3 |
PIK3R2 |
PPP3CC |
PTK2B |
SHC1 |
GAPDH |
|
C |
ADCY8 |
CALM2 |
CRK |
EGFR |
HRAS |
MAPK13 |
NPPB |
PIK3R3 |
PRKCA |
RAC1 |
SOS1 |
LDHA |
|
D |
AKT1 |
CAMK2A |
DGKB |
ELK1 |
ITPR1 |
MAPK14 |
NRAS |
PLA2G4A |
PRKCD |
RAF1 |
SOS2 |
NONO |
|
E |
AKT2 |
CAMK2B |
DGKE |
FOS |
ITPR2 |
MAPK3 |
NTRK1 |
PLA2G7 |
PRKCE |
RAP1A |
SP1 |
PPIH |
|
F |
ASIC1 |
CAMK2D |
ECE1 |
GNAI1 |
ITPR3 |
MAPK9 |
PIK3CA |
PLCG1 |
PTGER2 |
RAPGEF1 |
SRC |
GDC |
|
G |
ATF4 |
CAPN2 |
EDN1 |
GNAQ |
JUN |
MMP2 |
PIK3CB |
PLD1 |
PTGER4 |
RASGRP1 |
STAT5A |
PPC |
|
H |
CACNA1C |
CCND3 |
EDN3 |
GRB2 |
KRAS |
MT-CO2 |
PIK3CD |
PLD2 |
PTGS1 |
RHOA |
TRPV1 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK107 |
| 产地 | 美国 |
| 缩写 | GQH-EDN |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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