ScienCell's GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Kit (GQH-LRI) is designed to facilitate gene expression profiling of 40 selected lectin receptors that are involved in pathogen-specific immune responses. Brief examples of how included genes may be grouped according to their functions are shown below:
- C-Type Lectin Receptors: ASGR1, CD69, CD207, CD302, CLEC3B, CLEC4A-M, CLEC5A, CLEC6A, CLEC7A, CLEC10A, CLEC16A, LY75, MRC1, MRC2
- NK Cell lectin-like receptors: KLRB1, KLRC1, KLRC2, KLRD1, KLRK1
- Other subgroups such as selectins, chondrolectins and collectins: ACAN, CD72, CD93, CD248, MBL2, OLR1, PKD1, SELE, SELL, SELP, SFTPA2, SFTPD, THBD
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACAN |
CD93 |
CLEC4E |
KLRC1 |
OLR1 |
β-actin |
ACAN |
CD93 |
CLEC4E |
KLRC1 |
OLR1 |
β-actin |
|
B |
ASGR1 |
CLEC10A |
CLEC4M |
KLRC2 |
PKD1 |
GAPDH |
ASGR1 |
CLEC10A |
CLEC4M |
KLRC2 |
PKD1 |
GAPDH |
|
C |
CD207 |
CLEC12A |
CLEC5A |
KLRD1 |
SELE |
LDHA |
CD207 |
CLEC12A |
CLEC5A |
KLRD1 |
SELE |
LDHA |
|
D |
CD209 |
CLEC16A |
CLEC6A |
KLRK1 |
SELL |
NONO |
CD209 |
CLEC16A |
CLEC6A |
KLRK1 |
SELL |
NONO |
|
E |
CD248 |
CLEC3B |
CLEC7A |
LY75 |
SELP |
PPIH |
CD248 |
CLEC3B |
CLEC7A |
LY75 |
SELP |
PPIH |
|
F |
CD302 |
CLEC4A |
CLEC9A |
MBL2 |
SFTPA2 |
GDC |
CD302 |
CLEC4A |
CLEC9A |
MBL2 |
SFTPA2 |
GDC |
|
G |
CD69 |
CLEC4C |
FCER2 |
MRC1 |
SFTPD |
PPC |
CD69 |
CLEC4C |
FCER2 |
MRC1 |
SFTPD |
PPC |
|
H |
CD72 |
CLEC4D |
KLRB1 |
MRC2 |
THBD |
NTC |
CD72 |
CLEC4D |
KLRB1 |
MRC2 |
THBD |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK111 |
| 产地 | 美国 |
| 缩写 | GQH-LRI |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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