ScienCell's GeneQuery™ Human Neural Stem Cell Markers qPCR Array Kit (GQH-NSC) is designed to facilitate gene expression profiling of 40 marker genes commonly used to assess multipotent neural stem cell maintenance, proliferation, and differentiation.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Neural Stem Cell Markers qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ABCG2 |
PROM1 |
GFAP |
NUMB |
RXRA |
β-actin |
ABCG2 |
PROM1 |
GFAP |
NUMB |
RXRA |
β-actin |
|
B |
ASCL1 |
CDCP1 |
SLC2A1 |
OTX2 |
SOX1 |
GAPDH |
ASCL1 |
CDCP1 |
SLC2A1 |
OTX2 |
SOX1 |
GAPDH |
|
C |
CTNNB1 |
CXCR4 |
ID2 |
PAX3 |
SOX2 |
LDHA |
CTNNB1 |
CXCR4 |
ID2 |
PAX3 |
SOX2 |
LDHA |
|
D |
BMI1 |
FABP7 |
MSI1 |
PAX6 |
SOX9 |
NONO |
BMI1 |
FABP7 |
MSI1 |
PAX6 |
SOX9 |
NONO |
|
E |
SMARCA4 |
FGFR4 |
NES |
PDGFRA |
MSX1 |
PPIH |
SMARCA4 |
FGFR4 |
NES |
PDGFRA |
MSX1 |
PPIH |
|
F |
CDH2 |
FOXD3 |
NEUROD1 |
PRKCZ |
TRAF4 |
GDC |
CDH2 |
FOXD3 |
NEUROD1 |
PRKCZ |
TRAF4 |
GDC |
|
G |
CALCR |
FZD9 |
NOG |
ROR2 |
VIM |
PPC |
CALCR |
FZD9 |
NOG |
ROR2 |
VIM |
PPC |
|
H |
FUT4 |
GATA2 |
NOTCH1 |
RUNX1 |
ZIC1 |
NTC |
FUT4 |
GATA2 |
NOTCH1 |
RUNX1 |
ZIC1 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK113 |
| 产地 | 美国 |
| 缩写 | GQH-NSC |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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