ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:

• CLP markers: MME, CD34, IL7R, FLT3
• ProB-PreB-immature B cell markers: MME, MS4A1, SPN, PTPRC, IL7R, CD79A/B, CD40, CD19
• B1 cell markers: CD19, MS4A1, CD27, SPN, CD40LG
• Regulatory B cell markers: CD1D, CD5, CD19, CR2, CD24
• Marginal Zone B cell markers: CD1C, CD19, MS4A1, CR2, CD24
• Follicular B cell markers: CD19, MS4A1, CR2, CD22, FCER2
• Memory B cell markers: CD27, CD80, CD84, CD86
• Plasmablast-plasma cell markers: CD19, CD27, CD38, IL6R, SDC1, SLAMF7
• Double-negative and double-positive stage T cell markers: CD1A, CD3D/E/G, CD4, CD5, CD7, CD8A/B, CD38, SELE/L/P, CD160, CD200                       

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human B and T Cells Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

* gene selection may be updated based on new research and development