ScienCell's GeneQuery™ Human Keratocyte Biology qPCR Array (GQH-KER) profiles 88 key genes involved in corneal keratocyte function. Keratocytes are specialized fibroblasts that play roles in wound healing, maintaining corneal transparency, and corneal component synthesis. Keratocyte pathologies include keratoconus and limbal stem cell deficiency. Below are brief examples of how included genes may be grouped according to their function in keratocyte biology:
- Markers: MUC1, CD34, KERA, KRT19, LUM
- Wound Healing: MMP12, MRC1, NFKB1, COL3A1, CCL2
- Cell Cycling: MAPK1, MYCN, PTN, ETS1, CAPN9
- Oxidative Stress Response: RRAS, NOS2, NQO1, MAF, HMOX1
- Keratoconus Development: IGFBP3, MMP1, SSTR1, CRX, TNFAIP6
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Keratocyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTA1 |
CCL2 |
COL1A1 |
CRYAA |
F13A1 |
GSTK1 |
KERA |
MAPK3 |
NCAM1 |
PTN |
TGFBR1 |
β-actin |
|
B |
ACTA2 |
CCL7 |
COL1A2 |
CRYAB |
FASLG |
HMOX1 |
KRT1 |
MMP1 |
NFKB1 |
RRAS |
TGFBR2 |
GAPDH |
|
C |
ALDH1A1 |
CD34 |
COL3A1 |
CRYBB1 |
FGF2 |
ICAM1 |
KRT12 |
MMP12 |
NFKB2 |
SDC1 |
TIMP1 |
LDHA |
|
D |
ATF1 |
CD68 |
COL5A1 |
CRYGD |
FGFR1 |
IGF1R |
KRT19 |
MMP2 |
NOS2 |
SOD2 |
TIMP2 |
NONO |
|
E |
BGN |
CDK1 |
COL5A2 |
CXADR |
FGFR2 |
IGFBP3 |
LCN2 |
MMP3 |
NQO1 |
SPARC |
TIMP3 |
PPIH |
|
F |
BMP4 |
CFL1 |
COL6A2 |
ECM1 |
FN1 |
IGFBP5 |
LUM |
MRC1 |
PCNA |
SSTR1 |
TKT |
GDC |
|
G |
C12orf57 |
CHST6 |
COL7A1 |
EPHX1 |
FOS |
IL1A |
MAF |
MUC1 |
PDGFRB |
TC2N |
TNFAIP6 |
PPC |
|
H |
CAPN9 |
CLU |
CRX |
ETS1 |
FOSL1 |
IL6 |
MAPK1 |
MYCN |
PTGDS |
TGFB1 |
VCL |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK074 |
| 产地 | 美国 |
| 缩写 | GQH-KER |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK101 | 人类Synoviocyte细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK102 | 人类滋养层细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK105 | 人类ECM降解qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK106 | 人类焦点粘附复合物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK107 | 人内皮素信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK111 | 人凝集素调节免疫应答qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK113 | 人类神经干细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK114 | 人类小胶质细胞极化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK115 | 人类骨髓谱系标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK116 | 人B细胞和T细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK117 | 人胸腺细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK118 | 人类先天淋巴细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK119 | 人类神经祖细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK121 | 人PD-1 / PD-L1检查点途径qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK123 | 人类生长因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK124 | 人类转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK125 | 人干细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK126 | 人类细胞表面标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
