Preadipocytes are fibroblast-like precursor cells that can proliferate and differentiate into mature adipocytes. Preadipocytes play an important role in metabolic homeostasis and nutrient signaling. ScienCell's GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Kit (GQH-PAD) is designed to facilitate gene expression profiling of 88 key genes involved in human preadipocyte cell biology, such as cell proliferation, differentiation, ECM formation and lipid metabolism. Genes implicated in obesity and liposarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:
- Preadipocyte cell markers: ADIPOQ, LEP, HOXC8/9, TBX1, TMEM26, UCP1, CIDEA, PRDM16, SLC7A10, P2RX5
- Proliferation: CCND1, PROKR1, LYRM1, FTO, SLC2A4, PDGFA, IL4, IGF1, FGF5, MAPK1/3, TNFSF10
- Differentiation and adipogenesis: PPARG, CEBPA/B, KLF2/5/15, SOCS1/3, INSR, DLK1, ADD1, SREBF1
- ECM and cytoskeleton: MMP1/2/3, FAP, DPP4, TGFB1, COL1A1/2, SMAD2/4
- Lipid metabolism: FABP5/7, LBP, LPL, PLIN2, ACSL1, LIPC
- Obesity: MC4R, UCP3, ADRB2/3, POMC, FFAR4, CARTPT
- Liposarcoma: FUS, MDM2, HMGA2, DDIT3, CDK4, EWSR1, DES, VIM
Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Plate Layout *
(8 controls in Bold and Italic)
Note: all gene names follow their official symbols by HGNC
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ACSL1 |
CDK4 |
DES |
FAP |
HMGA2 |
KLF15 |
LTBP1 |
MMP3 |
PRDM16 |
SLC7A10 |
TMEM26 |
ACTB |
B |
ADD1 |
CEBPA |
DGAT2 |
FASLG |
HOXC8 |
KLF2 |
LYRM1 |
P2RX5 |
PROKR1 |
SMAD2 |
TNF |
GAPDH |
C |
ADIPOQ |
CEBPB |
DLK1 |
FFAR4 |
HOXC9 |
KLF5 |
MAPK1 |
PDGFA |
PTGR1 |
SMAD4 |
TNFSF10 |
LDHA |
D |
ADRB2 |
CIDEA |
DPP4 |
FGF5 |
IGF1 |
LBP |
MAPK3 |
PDGFRA |
RUNX2 |
SOCS1 |
UCP1 |
NONO |
E |
ADRB3 |
COL1A1 |
EWSR1 |
FTO |
IL4 |
LEP |
MC4R |
PLIN2 |
SDC3 |
SOCS3 |
UCP3 |
PPIH |
F |
AGRP |
COL1A2 |
FABP4 |
FUS |
INPPL1 |
LIPC |
MDM2 |
PNPLA2 |
SIM1 |
SREBF1 |
VIM |
GDC |
G |
CARTPT |
COL6A3 |
FABP5 |
GATA3 |
INSR |
LIPE |
MMP1 |
POMC |
SLC2A4 |
TBX1 |
WISP2 |
PPC |
H |
CCND1 |
DDIT3 |
FABP7 |
GLI3 |
KITLG |
LPL |
MMP2 |
PPARG |
SLC36A2 |
TGFB1 |
WNT5A |
NTC |
* gene selection may be updated based on new research and development
货号 | GK103 |
产地 | 美国 |
缩写 | GQH-PAD |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |
货号 | 产品名称 | 规格 | 价格 | 指令 | |
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GK102 | 人类滋养层细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK105 | 人类ECM降解qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK106 | 人类焦点粘附复合物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK107 | 人内皮素信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK111 | 人凝集素调节免疫应答qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK113 | 人类神经干细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK114 | 人类小胶质细胞极化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK115 | 人类骨髓谱系标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK116 | 人B细胞和T细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK117 | 人胸腺细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK118 | 人类先天淋巴细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK119 | 人类神经祖细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK121 | 人PD-1 / PD-L1检查点途径qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK123 | 人类生长因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK124 | 人类转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK125 | 人干细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK126 | 人类细胞表面标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |