Meningeal cells surround the brain and actively participate in the development of the central nervous system. They play an important role in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, and laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerebellar cortex and dentate gyrus. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex, in adult rat spinal cord lesions promotes axonal regrowth. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells. HMC from ScienCell Research Laboratories are isolated from human leptomeninges. HMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMC are characterized by immunofluorescence; they are positive for fibronectin and negative for GFAP and α-smooth muscle actin. HMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMC are guaranteed to further expand for 15 population doublings under the conditions specified by ScienCell Research Laboratories. Recommended Medium It is recommended to use Meningeal Cell Medium (MenCM, Cat. #1401) for culturing HMC in vitro.