The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK T-cells. Primary mouse splenocytes (MS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells. MS from ScienCell Research Laboratories are isolated from normal adult CD-1 mouse spleen. MS are depleted of splenic macrophages, cryopreserved directly after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. MS are quality control tested for viability. MS are negative for mycoplasma, bacteria, yeast, and fungi. MS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture. Recommended Medium It is recommended to use HematoGro Medium (HemGM, Cat. #5501) for short-term maintenance of MS in vitro.