人端粒长度和线粒体DNA拷贝数双重分析定量qPCR分析试剂盒（绝对定量）AHDQ
Absolute Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification qPCR Assay Kit
品牌：
ScienCell
货号：
8958
价格：
$556
产品说明书下载：
产品预定
>
产品预定，提交订单信息之后，我们会第一时间与您取得联系
{{item}}
Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions and oncogenesis. Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in the energy production. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Evidence suggests that telomere length and mitochondrial dysfunction are positively correlated in cellular aging and other age-related disorders such as cancer, diabetes and neurodegenerative diseases. A possible mechanistic link between them has been proposed through the PPAR signaling pathway. However, the specifics of the mechanism still has to be elucidated. ScienCell's Absolute Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification qPCR Assay Kit (AHDQ) is designed to simultaneously quantify the average telomere length and mtDNA copy number of a human cell population using qPCR. The telomere primer set recognizes and amplifies telomere sequences. The mtDNA primer set recognizes and amplifies one of the most conserved regions on human mtDNA and will not amplify any off-target sequence on nuclear genomic DNA. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on human chromosome 17, and serves as reference for data normalization. The reference genomic DNA sample with known telomere length and mtDNA copy number serves as a reference for calculating the telomere length and the mtDNA copy number of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.
| 货号 | 8958 |
| 产地 | 美国 |
| 缩写 | AHDQ |
| 规格 | 100 reactions |
| 用途 | 科研 |
| 储存 | -20度 |
| 运输 | 干冰 |
Publications Promotion
Has your paper been published using one or more of Sciencell products? Get rewarded! Receive 10% off one order*. See details >>
1.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.
2.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.
3.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197
4.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184
5.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40
There are no product questions yet.
细胞检测试剂盒
细胞检测试剂盒
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| 0223 | 茜素红S染色试剂盒 ARed | 100ml | $86 | + | |
| 0833 | 人胚层鉴定试剂盒 HGL PCR | 50 reactions | $374 | + | |
| 0843 | 油红O染色试剂盒 OEed | 100 ml/bottle - 2 bottles | $110 | + | |
| 0853 | 人干细胞多能性检测试剂盒 HSC PCR | 50 reactions | $374 | + | |
| 3D-4110 | 3D肾小管上皮细胞形成试剂盒 3D-RTF | Kit | $1054 | + | |
| 8008 | I型胶原细胞粘附检测试剂盒 | 48 tests | $273 | + | |
| 8018 | 纤维粘连细胞粘附检测试剂盒 | 48 tests | $273 | + | |
| 8028 | MTT细胞活力和增殖检测试剂盒 | 1000 tests in 96-well plate | $160 | + | |
| 8038 | WST-1细胞活力和增值检测试剂盒 | 1000 tests in 96-well plate | $273 | + | |
| 8058 | 细胞衰老检测试剂盒 | 50 tests/35 mm plate | $273 | + | |
| 8068 | β-半乳糖苷酶比色检测试剂盒 GalC | 50 tests/35 mm plate | $273 | + | |
| 8078 | LDH细胞毒性检测试剂盒 LDH | 500 tests | $226 | + | |
| 8088 | TUNEL色标记细胞凋亡检测试剂盒 | 50 tests | $477 | + | |
| 8098 | 一氧化氮比色法检测试剂盒 NO | 250 tests | $316 | + | |
| 8108 | 磷酸酶检测试剂盒 pNPP | 500 tests | $137 | + | |
| 8118 | 孔雀绿磷酸盐检测试剂盒 MGPA | 2500 tests | $137 | + | |
| 8128 | 比色钙法分析试剂盒 CCA | 250 tests | $240 | + | |
| 8138 | 活/死细胞染色试剂盒 | 1000 tests | $354 | + | |
| 8148 | 比色法GAPDH分析试剂盒 GAPDH | 100 tests | $149 | + | |
| 8158 | 体外管腔形成分析试剂盒 | 50 tests | $348 | + | |
| 8168 | 总抗氧化能力检测试剂盒 TACA | 100 tests | $297 | + | |
| 8178 | 胶原 I-3D凝胶试剂盒 | 100 mg | $187 | + | |
| 8198 | 超氧化物歧化酶分析试剂盒 SOD | 100 tests | $137 | + | |
| 8198 | 超氧化物歧化酶分析试剂盒 SOD | 100 tests | $137 | + | |
| 8208 | 支原体PCR检测试剂盒 | 100 Tests | $292 | + | |
| 8218 | 过氧化氢酶活性分析试剂盒 CAT | 100 tests | $273 | + | |
| 8218 | 过氧化氢酶活性分析试剂盒 CAT | 100 tests | $273 | + | |
| 8228 | 细胞凋亡蛋白酶-3分析试剂盒 | 100 tests | $354 | + | |
| 8238 | 谷胱甘肽过氧化氢酶检测试剂盒 GPx | 100 tests | $374 | + | |
| 8238 | 谷胱甘肽过氧化氢酶检测试剂盒 GPx | 100 tests | $374 | + |
